iTRAQ - Isobaric tags for relative and absolute quantitation
Labelling with isobaric tags enables the incorporation of a stable isotope chemical modification onto trypsin digested peptides. We have extensive experience of isobaric tag projects investigating cell culture monolayers and 3D spheroid models, preclinical models, tissue and liquid biopsies. Human biopsies can be investigated individually comparing matched healthy and disease material, or for large cohorts (with extracts from patients in the same group pooled to identify common expression changes).
Our Proteomics Service includes full consultation on the design of 4-, 6-, 8- and 10-plex isobaric label-based experimental strategies.