Studying TDP1 function in DNA repair
Chiang S.;Liversidge K.;El-Khamisy S. (2018) Methods in Molecular Biology. DNA repair. 173-181.
I am a pharmacist interested in understanding how cells maintain genomic integrity. I use interdisciplinary approach fusing genetics, cell biology, biochemistry and spectroscopy with clinical expertise. To further strengthen our translational agenda, I recently took up a joint appointment between the University of Sheffield and the University of Bradford to head the Institute of Cancer Therapeutics. My contributions led to discovery of novel genetic pathways, influenced healthcare practice and developed new technologies. My early work demonstrated a role for PARP1 and CK2 during chromosomal single-strand break (SSB) repair (NAR 2003, Cell 2004). I then moved into more translational aspects, which led to the identification, for the first time, of a defect in SSB repair in two human disorders (Nature 2005 and 2006). Following training at St Jude Hospital in the US, I employed mice to demonstrate the importance of SSB repair for organismal function (EMBO J 2007 and Nature Neuroscience 2009). My interest in this area led to a search for novel activities that repair topoisomerase-linked DNA breaks and resulted in the discovery of the enzyme that repairs topoisomerase 2 - mediated DNA damage, TDP2 (Nature 2009). This seminal finding is critical for a broad range of human disease including cancer. I demonstrated that topoisomerase-induced DNA breaks are pathogenic in other disorders (Hum Mol Gent 2010, Nature Genetics 2014, Nature Neuroscience 2017) and identified key posttranslational modifications and functions (Nature Communications 2012, NAR 2012 & 2016 and Cell Reports 2018). I reviewed the importance of topoisomerase-mediated DNA repair (Nature Rev Cancer 2015, Brain 2018) and described how it maintains mitochondrial gene transcription (Science Advances 2017). We uncovered a novel mechanism by which typhoid toxin exhausts the DNA repair machinery (Nature Communications 2019) and identified TEX264 as a novel component of the topoisomerase-mediated DNA repair pathway (Nature Communications 2020).
For lab details please visit: https://www.elkhamisylab.com
Chiang S.;Liversidge K.;El-Khamisy S. (2018) Methods in Molecular Biology. DNA repair. 173-181.
Allam W.;Ashour M.;Waly A.;El-Khamisy S. (2017) Advances in Experimental Medicine and Biology. DNA repair . 41-58.
Kim HR;Santhakumar K;Markham E;Baldera D;Greenald D;Bryant HE;El-Khamisy SF;van Eeden FJ; (2020) Oncotarget. 11
Halliwell JA;Frith TJR;Laing O;Price CJ;Bower OJ;Stavish D;Gokhale PJ;Hewitt Z;El-Khamisy SF;Barbaric I;Andrews PW; (2020) Stem Cell Reports. 14
Fielden J;Wiseman K;Torrecilla I;Li S;Hume S;Chiang SC;Ruggiano A;Narayan Singh A;Freire R;Hassanieh S;Domingo E;Vendrell I;Fischer R;Kessler BM;Maughan TS;El-Khamisy SF;Ramadan K; (2020) Nature Communications. 11
Ibler AEM;ElGhazaly M;Naylor KL;Bulgakova NA;F El-Khamisy S;Humphreys D; (2019) Nature Communications. 10
Osman DI;El-Sheikh SM;Sheta SM;Ali OI;Salem AM;Shousha WG;El-Khamisy SF;Shawky SM; (2019) Biosensors and Bioelectronics. 141
Abugable AA;Morris JLM;Palminha NM;Zaksauskaite R;Ray S;El-Khamisy SF; (2019) DNA Repair. 81
Elserafy M;El-Khamisy SF; (2018) Nature Reviews Molecular Cell Biology. 19
Mohamed Z;Hassan MK;Okasha S;Mitamura T;Keshk S;Konno Y;Kato T;El-Khamisy SF;Ohba Y;Watari H; (2018) Oncotarget. 9
Moradzadeh M;Ghorbani A;Erfanian S;Mohaddes ST;Rahimi H;Karimiani EG;Mashkani B;Chiang SC;El-Khamisy SF;Tabarraei A;Sadeghnia HR; (2018) Journal of Cellular Biochemistry.
Fleifel D;Rahmoon MA;AlOkda A;Nasr M;Elserafy M;El-Khamisy SF; (2018) Genetic Engineering and Biotechnology Journal. 16
Hegazy MT;Allam WR;Hussein MA;Zoheir N;Quartuccio L;El-Khamisy SF;Ragab G; ; (2018) Ebiomedicine. 35
Elserafy M;Abugable AA;Atteya R;El-Khamisy SF; (2018) Trends in Genetics. 34
Southam HM;Smith TW;Lyon RL;Liao C;Trevitt CR;Middlemiss LA;Cox FL;Chapman JA;El-Khamisy SF;Hippler M;Williamson MP;Henderson PJF;Poole RK; (2018) Redox Biology. 18
Shawky SM;Awad AM;Abugable AA;El-Khamisy SF; (2018) International journal of nanomedicine. 13
Walker C;El-Khamisy SF; (2018) Brain. 141
Samir N;Matboli M;El-Tayeb H;El-Tawdi A;Hassan MK;Waly A;El-Akkad HAE;Ramadan MG;Al-Belkini TN;El-Khamisy S;El-Asmar F; (2018) Journal of Cellular Biochemistry. 119
Kassouf N;Kay CWM;Volkov A;Chiang SC;Birch-Machin MA;El-Khamisy SF;Haywood RM; (2018) Free Radical Biology and Medicine. 126
Liao C;Beveridge R;Hudson JJR;Parker JD;Chiang SC;Ray S;Ashour ME;Sudbery I;Dickman MJ;El-Khamisy SF; (2018) Cell Reports. 23
Chiang SC;Liversidge K;El-Khamisy SF; (2018) Methods in molecular biology (Clifton, N.J.). 1703
Shawky SM;Awad AM;Allam W;Alkordi MH;El-Khamisy SF; (2017) Biosensors and Bioelectronics. 92
Abugable AA;Awwad DA;Fleifel D;Ali MM;El-Khamisy S;Elserafy M; (2017) Advances in Experimental Medicine and Biology. 1007
Atteya R;Ashour ME;Ibrahim EE;Farag MA;El-Khamisy SF; (2017) Mechanisms of Ageing and Development. 161
Chiang SC;Meagher M;Kassouf N;Hafezparast M;McKinnon PJ;Haywood R;El-Khamisy SF; (2017) Science Advances. 3
Walker C;Herranz-Martin S;Karyka E;Liao C;Lewis K;Elsayed W;Lukashchuk V;Chiang SC;Ray S;Mulcahy PJ;Jurga M;Tsagakis I;Iannitti T;Chandran J;Coldicott I;De Vos KJ;Hassan MK;Higginbottom A;Shaw PJ;Hautbergue GM;Azzouz M;El-Khamisy SF; (2017) Nature Neuroscience. 20
Herranz-Martin S;Chandran J;Lewis K;Mulcahy P;Higginbottom A;Walker C;Valenzuela IMY;Jones RA;Coldicott I;Iannitti T;Akaaboune M;El-Khamisy SF;Gillingwater TH;Shaw PJ;Azzouz M; (2017) Disease models & mechanisms. 10
Hautbergue GM;Castelli LM;Ferraiuolo L;Sanchez-Martinez A;Cooper-Knock J;Higginbottom A;Lin YH;Bauer CS;Dodd JE;Myszczynska MA;Alam SM;Garneret P;Chandran JS;Karyka E;Stopford MJ;Smith EF;Kirby J;Meyer K;Kaspar BK;Isaacs AM;El-Khamisy SF;De Vos KJ;Ning K;Azzouz M;Whitworth AJ;Shaw PJ; (2017) Nature Communications. 8
Meisenberg C;Ashour ME;El-Shafie L;Liao C;Hodgson A;Pilborough A;Khurram SA;Downs JA;Ward SE;El-Khamisy SF; (2017) Nucleic Acids Research. 45
Lissek T;Adams M;Adelman J;Ahissar E;Akaaboune M;Akil H;al'Absi M;Arain F;Arango-Lasprilla JC;Atasoy D;Avila J;Badawi A;Bading H;Baig AM;Baleriola J;Belmonte C;Bertocchi I;Betz H;Blakemore C;Blanke O;Boehm-Sturm P;Bonhoeffer T;Bonifazi P;Brose N;Campolongo P;Celikel T;Chang CC;Chang TY;Citri A;Cline HT;Cortes JM;Cullen K;Dean K;Delgado-Garcia JM;Desroches M;Disterhoft JF;Dowling JE;Draguhn A;El-Khamisy SF;El Manira A;Enam SA;Encinas JM;Erramuzpe A;Esteban JA;Fariñas I;Fischer E;Fukunaga I;Gabilondo I;Ganten D;Gidon A;Gomez-Esteban JC;Greengard P;Grinevich V;Gruart A;Guillemin R;Hariri AR;Hassan B;Häusser M;Hayashi Y;Hussain NK;Jabbar AA;Jaber M;Jahn R;Janahi EM;Kabbaj M;Kettenmann H;Kindt M;Knafo S;Köhr G;Komai S;Krugers H;Kuhn B;Ghazal NL;Larkum ME;London M;Lutz B;Matute C;Martinez-Millan L;Maroun M;McGaugh J;Moustafa AA;Nasim A;Nave KA;Neher E;Nikolich K;Outeiro T;Palmer LM;Penagarikano O;Perez-Otano I;Pfaff DW;Poucet B;Rahman AU;Ramos-Cabrer P;Rashidy-Pour A;Roberts RJ;Rodrigues S;Sanes JR;Schaefer AT;Segal M;Segev I;Shafqat S;Siddiqui NA;Soreq H;Soriano-García E;Spanagel R;Sprengel R;Stuart G;Südhof TC;Tønnesen J;Treviño M;Uthman BM;Venter JC;Verkhratsky A;Weiss C;Wiesel TN;Yaksi E;Yizhar O;Young LJ;Young P;Zawia NH;Zugaza JL;Hasan MT; (2017) Neuron. 96
Elsayed W;El-Shafie L;Hassan MK;Farag MA;El-Khamisy SF; (2016) Scientific Reports. 6
Mazouzi A;Stukalov A;Müller AC;Chen D;Wiedner M;Prochazkova J;Chiang SC;Schuster M;Breitwieser FP;Pichlmair A;El-Khamisy SF;Bock C;Kralovics R;Colinge J;Bennett KL;Loizou JI; (2016) Cell Reports. 15
Hassan MK;Watari H;Mitamura T;Mohamed Z;El-Khamisy SF;Ohba Y;Sakuragi N; (2015) ONCOSCIENCE. 2
Meisenberg C;Gilbert DC;Chalmers A;Haley V;Gollins S;Ward SE;El-Khamisy SF; (2015) Molecular Cancer Therapeutics. 14
Ashour ME;Atteya R;El-Khamisy SF; (2015) Nature Reviews. Cancer. 15
Carroll J;Page TK;Chiang SC;Kalmar B;Bode D;Greensmith L;Mckinnon PJ;Thorpe JR;Hafezparast M;El-Khamisy SF; (2015) Human Molecular Genetics. 24
Walker S;Meisenberg C;Bibby RA;Askwith T;Williams G;Rininsland FH;Pearl LH;Oliver AW;El-Khamisy S;Ward S;Atack JR; (2014) Analytical Biochemistry. 454
Gómez-Herreros F;Schuurs-Hoeijmakers JH;McCormack M;Greally MT;Rulten S;Romero-Granados R;Counihan TJ;Chaila E;Conroy J;Ennis S;Delanty N;Cortés-Ledesma F;de Brouwer AP;Cavalleri GL;El-Khamisy SF;de Vries BB;Caldecott KW; (2014) Nature Genetics. 46
Meisenberg C;Ward SE;Schmid P;El-Khamisy SF; (2014) Journal of Cancer Science and Therapy. 6
Alagoz M;Wells OS;El-Khamisy SF; (2014) Nucleic Acids Research. 42
Alagoz M;Chiang SC;Sharma A;El-Khamisy SF; (2013) PLoS ONE. 8
Gilbert DC;Chalmers AJ;El-Khamisy SF; (2012) British Journal of Cancer. 106
Lebedeva NA;Rechkunova NI;El-Khamisy SF;Lavrik OI; (2012) BIOCHIMIE. 94
Alagoz M;Gilbert DC;El-Khamisy S;Chalmers AJ; (2012) Current Medicinal Chemistry. 19
Zeng Z;Sharma A;Ju L;Murai J;Umans L;Vermeire L;Pommier Y;Takeda S;Huylebroeck D;Caldecott KW;El-Khamisy SF; (2012) Nucleic Acids Research. 40
Hudson JJ;Chiang SC;Wells OS;Rookyard C;El-Khamisy SF; (2012) Nature Communications. 3
Zeng Z;Cortés-Ledesma F;El Khamisy SF;Caldecott KW; (2011) Journal of Biological Chemistry. 286
El-Khamisy SF; (2011) EMBO Molecular Medicine. 3
Chiang SC;Carroll J;El-Khamisy SF; (2010) Cell Cycle. 9
Kerzendorfer C;Whibley A;Carpenter G;Outwin E;Chiang SC;Turner G;Schwartz C;El-Khamisy S;Raymond FL;O'Driscoll M; (2010) Human Molecular Genetics. 19
El-Khamisy SF;Katyal S;Patel P;Ju L;McKinnon PJ;Caldecott KW; (2009) DNA Repair. 8
Cortes Ledesma F;El Khamisy SF;Zuma MC;Osborn K;Caldecott KW; (2009) Nature. 461
Reynolds JJ;El-Khamisy SF;Caldecott KW; (2009) Biochemical Society Transactions. 37
Lee Y;Katyal S;Li Y;El-Khamisy SF;Russell HR;Caldecott KW;McKinnon PJ; (2009) Nature Neuroscience. 12
Reynolds J.;El-Khamisy S.;Katyal S.;Clements P.;McKinnon P.;Caldecott K. (2009) Molecular and Cellular Biology. 29, 1354-1362.
El-Khamisy SF;Caldecott KW; (2007) Neuroscience. 145
Iles N;Rulten S;El-Khamisy SF;Caldecott KW; (2007) Molecular and Cellular Biology. 27
Katyal S;el-Khamisy SF;Russell HR;Li Y;Ju L;Caldecott KW;McKinnon PJ; (2007) EMBO Journal. 26
El-Khamisy SF;Hartsuiker E;Caldecott KW; (2007) DNA Repair. 6
Breslin C.;Clements P.;El-Khamisy S.;Petermann E.;Iles N.;Caldecott K. (2006) Methods in Enzymology. 409, 410-425.
El-Khamisy SF;Caldecott KW; (2006) Mutagenesis. 21
Ahel I;Rass U;El-Khamisy SF;Katyal S;Clements PM;McKinnon PJ;Caldecott KW;West SC; (2006) Nature. 443
El-Khamisy SF;Saifi GM;Weinfeld M;Johansson F;Helleday T;Lupski JR;Caldecott KW; (2005) Nature. 434
Loizou J.;El-Khamisy S.;Zlatanou A.;Moore D.;Chan D.;Qin J.;Sarno S.;Meggio F.;Pinna L.;Caldecott K. (2004) Cell. 117, 17-28.
El-Khamisy SF;Masutani M;Suzuki H;Caldecott KW; (2003) Nucleic Acids Research. 31
Abugable A.A.;Morris J.L.M.;Palminha N.M.;Zaksauskaite R.;Ray S.;El-Khamisy S.F. (2019) DNA Repair.
Osman D.I.;El-Sheikh S.M.;Sheta S.M.;Ali O.I.;Salem A.M.;Shousha W.G.;EL-Khamisy S.F.;Shawky S.M. (2019) Biosensors and Bioelectronics.
Walker C.;El-Khamisy S. (2018) Brain.
Gilbert D.;Chalmers A.;El-Khamisy S. (2012) British Journal of Cancer.
El-Khamisy S. (2011) EMBO Molecular Medicine.
El-Khamisy S.;Caldecott K. (2007) Neuroscience.
El-Khamisy S.;Caldecott K. (2006) Mutagenesis.