Dr Refaat Hamed
Assistant Professor
| Faculty/Dept/School |
School of Chemistry and Bioscience (Faculty of Life Sciences) |
|---|---|
| r.farghal@bradford.ac.uk | |
| Telephone | +441274 232371 |
Biography
I have always been fascinated by the way nature morphs simple building blocks to create more complex natural cures. This fascination shaped my career, which started by the isolation, structure elucidation and biological evaluation of various classes of phytochemicals, with the focus being iridoid glycosides (MSc studies). I then moved on to pursue my D.Phil. studies at University of Oxford, where I learned the modern aspects of (b-lactam antibiotics) biosynthesis, molecular biology and enzymology (structural and mechanistic). During my D.Phil. and postdoctoral studies time, I have developed myself into a rational-design protein engineer. Challenging chemical transformations and forcing enzymes, via engineering, to catalyse these reactions, with exquisite regio- and stereoselectivity, for biocatalytic purposes, is one of the main themes of my research. Extending my research from engineering enzymes to redesign of biosynthetic pathways to produce unnatural products of pharmaceutical interest is another objective for my research. As a teacher, I do strive to be well-informed, interactive and inspiring. One of my primary goals of teaching is to prepare both undergraduate and graduate students for a successful career.
Research
Natural products discovery, Biosynthesis of natural products, Biocatalysis, Enzymology, Protein Engineering and Synthetic Biology| Date | Role | Title / Description | Funder | Award |
|---|---|---|---|---|
| 01-FEB-19 - 31-JAN-22 | Maximising Metabolic Efficiency for Cellular Alkyl Methacrylate Production Maximising Metabolic Efficiency for Cellular Alkyl Methacrylate Production | Biotechnology and Biological Sciences Research Council |
Teaching
Teaching interests
Organic Chemistry, Medicinal Chemistry, Biochemistry
Teaching highlights
NMR Spectroscopy
Carbonyl Chemistry
Stereoselective Synthesis
Retrosynthesis
Professional activities
- 01-JAN-10: University of Oxford - DPhil Organic Chemistry
- 01-JAN-04: Assiut University - MSc in Natural Products Chemistry
- 01-JAN-99: Assiut University - BSc in Pharmaceutical Sciences (with highest honors)
- 01-JUL-16: Assiut University, Egypt - Associate Professor (On leave)
- 01-JUN-11: Assiut University, Egypt - Assistant Professor
- 01-APR-04: Assiut University, Egypt - Assistant lecturer
- 01-DEC-99: Assiut University, Egypt - Demonstrator
- 27-FEB-18: University of Bradford - Lecturer of Chemical Biology
- 16-JAN-16: University of St Andrews - Research Fellow
- 30-MAY-13: University of Illinois Urbana Champaign - Research Fellow
- 10-MAR-10: University of Oxford - Research Fellow
- 01-APR-19: British Society for Antimicrobial Chemotherapy, Member of British Society for Antimicrobial Chemotherapy
- 01-DEC-18: American Chemical Society, Member of American Chemical Society
- 26-OCT-18: Biochemical Society, Member of Biochemical Society
- 28-JAN-11: Royal Society of Chemistry, Member of Royal Society of Chemistry
- 01-JAN-17: Assiut University Encouragement Award
- 29-APR-10: PCT/GB2009/051435 - Methods for preparing heterocyclic rings
Publications
| Title | Studies on the selectivity of proline hydroxylases reveal new substrates including bicycles (2019) |
|---|---|
| Authors | Tristan J. Smart, Refaat B. Hamed, Timothy D.W. Claridge, Christopher J. Schofield |
| Journal | Bioorganic Chemistry |
| DOI | 10.1016/j.bioorg.2019.103386 |
| Title | Biocatalytic production of bicyclic β-lactams with three contiguous chiral centres using engineered crotonases (2019) |
| Authors | Hamed, Refaat B.; Gomez-Castellanos, J.R.; Warhaut, H.L.; Claridge, T.D.W.; Schofield, C.J. |
| Journal | Communications Chemistry |
| Title | A natural solution to photoprotection and isolation of the potent polyene antibiotic, marinomycin A (2019) |
| Authors | Christopher S. Bailey, Joseph S. Zarins-Tutt, Matthias Agbo, Hong Gao, Alberto Diego-Taboada, Maoluo Gan, Refaat B. Hamed, Emily R. Abraham, Grahame Mackenzie, P. Andrew Evans, Rebecca J. M. Goss; |
| Journal | Chemical Science |
| DOI | 10.1039/C9SC01375J |
| Title | Stereoselective production of dimethyl-substituted carbapenams via engineered carbapenem biosynthesis enzymes (2017) |
| Authors | Hamed, Refaat B.; Henry, L.; Claridge, T.D.W.; Schofield, C. |
| Journal | ACS Catalysis |
| Title | Living GenoChemetics by hyphenating synthetic biology and synthetic chemistry in vivo (2017) |
| Authors | Sharma, S.V.; Tong, X.; Pubill-Ulldemolins, C.; Cartmell, C.; Bogosyan, E.J.A.; Rackham, E.J.; Marelli, E.; Hamed, Refaat B.; Goss, R.J.M. |
| Journal | Nature Communications |
| Title | Crotonases: Nature’s exceedingly convertible catalysts (2017) |
| Authors | Lohans, C.T.; Wang, D.Y.; Wang, J.; Hamed, Refaat B.; Schofield, C.J. |
| Journal | ACS Catalysis |
| Title | Use of Methylmalonyl-CoA Epimerase in Enhancing Crotonase Stereoselectivity (2016) |
| Authors | Hamed, Refaat B.; Gomez-Castellanos, J.R.; Sean Froese, D.; Krysztofinska, E.; Yue, W.W.; Schofield, C.J. |
| Journal | ChemBioChem |
| Title | Stereoselective preparation of lipidated carboxymethyl-proline/pipecolic acid derivatives via coupling of engineered crotonases with an alkylmalonyl-CoA synthetase (2013) |
| Authors | Hamed, R.B.;Henry, L.;Gomez-Castellanos, J.R.;Asghar, A.;Brem, J.;Claridge, T.D.W.;Schofield, C.J.; |
| Journal | Organic and Biomolecular Chemistry |
| DOI | 10.1039/c3ob41525b |
| Title | The enzymes of β-lactam biosynthesis (2013) |
| Authors | Hamed, R.B.;Gomez-Castellanos, J.R.;Henry, L.;Ducho, C.;McDonough, M.A.;Schofield, C.J.; |
| Journal | Natural Product Reports |
| DOI | 10.1039/c2np20065a |
| Title | Crotonase catalysis enables flexible production of functionalized prolines and carbapenams (2012) |
| Authors | Hamed, R.B.;Henry, L.;Gomez-Castellanos, J.R.;MecinoviÄ, J.;Ducho, C.;Sorensen, J.L.;Claridge, T.D.W.;Schofield, C.J.; |
| Journal | Journal of the American Chemical Society |
| DOI | 10.1021/ja208318d |
| Title | Oxygenase-catalyzed ribosome hydroxylation occurs in prokaryotes and humans (2012) |
| Authors | Ge, W.;Wolf, A.;Feng, T.;Ho, C.-H.;Sekirnik, R.;Zayer, A.;Granatino, N.;Cockman, M.E.;Loenarz, C.;Loik, N.D.;Hardy, A.P.;Claridge, T.D.W.;Hamed, R.B.;Chowdhury, R.;Gong, L.;Robinson, C.V.;Trudgian, D.C.;Jiang, M.;MacKeen, M.M.;McCullagh, J.S.;Gordiyenko, Y.;Thalhammer, A.;Yamamoto, A.;Yang, M.;Liu-Yi, P.;Zhang, Z.;Schmidt-Zachmann, M.;Kessler, B.M.;Ratcliffe, P.J.;Preston, G.M.;Coleman, M.L.;Schofield, C.J.; |
| Journal | Nature Chemical Biology |
| DOI | 10.1038/nchembio.1093 |
| Title | Self-hydroxylation of the splicing factor lysyl hydroxylase, JMJD6 (2012) |
| Authors | Mantri, M.;Webby, C.J.;Loik, N.D.;Hamed, R.B.;Nielsen, M.L.;McDonough, M.A.;McCullagh, J.S.O.;Böttger, A.;Schofield, C.J.;Wolf, A.; |
| Journal | MedChemComm |
| DOI | 10.1039/c1md00225b |
| Title | Photoactivable peptides for identifying enzyme-substrate and protein-protein interactions (2011) |
| Authors | Rotili, D.;Altun, M.;Hamed, R.B.;Loenarz, C.;Thalhammer, A.;Hopkinson, R.J.;Tian, Y.-M.;Ratcliffe, P.J.;Mai, A.;Kessler, B.M.;Schofield, C.J.; |
| Journal | Chemical Communications |
| DOI | 10.1039/c0cc04457a |
| Title | Factor-inhibiting hypoxia-inducible factor (FIH) catalyses the post-translational hydroxylation of histidinyl residues within ankyrin repeat domains (2011) |
| Authors | Yang, M.;Chowdhury, R.;Ge, W.;Hamed, R.B.;McDonough, M.A.;Claridge, T.D.W.;Kessler, B.M.;Cockman, M.E.;Ratcliffe, P.J.;Schofield, C.J.; |
| Journal | FEBS Journal |
| DOI | 10.1111/j.1742-4658.2011.08022.x |
| Title | Stereoselective C-C bond formation catalysed by engineered carboxymethylproline synthases (2011) |
| Authors | Hamed, R.B.;Gomez-Castellanos, J.R.;Thalhammer, A.;Harding, D.;Ducho, C.;Claridge, T.D.W.;Schofield, C.J.; |
| Journal | Nature Chemistry |
| DOI | 10.1038/nchem.1011 |
| Title | The 2-Oxoglutarate-Dependent Oxygenase JMJD6 Catalyses Oxidation of Lysine Residues to give 5S-Hydroxylysine Residues (2011) |
| Authors | Mantri, M.;Loik, N.D.;Hamed, R.B.;Claridge, T.D.W.;McCullagh, J.S.O.;Schofield, C.J.; |
| Journal | ChemBioChem |
| DOI | 10.1002/cbic.201000641 |
| Title | Monitoring the activity of 2-oxoglutarate dependent histone demethylases by NMR spectroscopy: Direct observation of formaldehyde (2010) |
| Authors | Hopkinson, R.J.;Hamed, R.B.;Rose, N.R.;Claridge, T.D.W.;Schofield, C.J.; |
| Journal | ChemBioChem |
| DOI | 10.1002/cbic.200900713 |
| Title | Carboxymethylproline synthase catalysed syntheses of functionalised N-heterocycles (2010) |
| Authors | Hamed, R.B.;MecinoviÄ, J.;Ducho, C.;Claridge, T.D.W.;Schofield, C.J.; |
| Journal | Chemical Communications |
| DOI | 10.1039/b924519g |
| Title | Evidence for the slow reaction of hypoxia-inducible factor prolyl hydroxylase 2 with oxygen (2010) |
| Authors | Flashman, E.;Hoffart, L.M.;Hamed, R.B.;Bollinger Jr., J.M.;Krebs, C.;Schofield, C.J.; |
| Journal | FEBS Journal |
| DOI | 10.1111/j.1742-4658.2010.07804.x |
| Title | Evidence that thienamycin biosynthesis proceeds via C-5 epimerization: ThnE catalyzes the formation of (2S,5S)-trans-carboxymethylproline (2009) |
| Authors | Hamed, R.B.;Batchelar, E.T.;MecinoviÄ, J.;Claridge, T.D.W.;Schofield, C.J.; |
| Journal | ChemBioChem |
| DOI | 10.1002/cbic.200800652 |
| Title | Synthesis of regio- and stereoselectively deuterium-labelled derivatives of l-glutamate semialdehyde for studies on carbapenem biosynthesis (2009) |
| Authors | Ducho, C.;Hamed, R.B.;Batchelar, E.T.;Sorensen, J.L.;Odell, B.;Schofield, C.J.; |
| Journal | Organic and Biomolecular Chemistry |
| DOI | 10.1039/b903312b |
| Title | Iron-mediated cleavage of C-C bonds in vicinal tricarbonyl compounds in water (2009) |
| Authors | MecinoviÄ, J.;Hamed, R.B.;Schofield, C.J.; |
| Journal | Angewandte Chemie - International Edition |
| DOI | 10.1002/anie.200806296 |
| Title | Mechanisms and structures of crotonase superfamily enzymes - How nature controls enolate and oxyanion reactivity (2008) |
| Authors | Hamed, R.B.;Batchelar, E.T.;Clifton, I.J.;Schofield, C.J.; |
| Journal | Cellular and Molecular Life Sciences |
| DOI | 10.1007/s00018-008-8082-6 |
| Title | Thioester hydrolysis and C-C bond formation by carboxymethylproline synthase from the crotonase superfamily (2008) |
| Authors | Batchelar, E.T.;Hamed, R.B.;Ducho, C.;Claridge, T.D.W.;Edelmann, M.J.;Kessler, B.;Schofield, C.J.; |
| Journal | Angewandte Chemie - International Edition |
| DOI | 10.1002/anie.200803906 |