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Dr. Refaat Hamed,
Assistant Professor

Information about Dr. Refaat Hamed at the University of Bradford.

School of Chemistry & Biosciences
(Faculty of Life Sciences)
Email:
r.farghal@bradford.ac.uk
Telephone:
+44 1274 232371

Biography

I have always been fascinated by the way nature morphs simple building blocks to create more complex natural cures. This fascination shaped my career, which started by the isolation, structure elucidation and biological evaluation of various classes of phytochemicals, with the focus being iridoid glycosides (MSc studies). I then moved on to pursue my D.Phil. studies at University of Oxford, where I learned the modern aspects of (b-lactam antibiotics) biosynthesis, molecular biology and enzymology (structural and mechanistic). During my D.Phil. and postdoctoral studies time, I have developed myself into a rational-design protein engineer. Challenging chemical transformations and forcing enzymes, via engineering, to catalyse these reactions, with exquisite regio- and stereoselectivity, for biocatalytic purposes, is one of the main themes of my research. Extending my research from engineering enzymes to redesign of biosynthetic pathways to produce unnatural products of pharmaceutical interest is another objective for my research. As a teacher, I do strive to be well-informed, interactive and inspiring. One of my primary goals of teaching is to prepare both undergraduate and graduate students for a successful career.

Research

Natural products discovery, Biosynthesis of natural products, Biocatalysis, Enzymology, Protein Engineering and Synthetic Biology

Research projects

Research projects involving Dr. Refaat Hamed.
Role Date Title/description Funder Award
2019-02-01T00:00:00 http://gtr.rcuk.ac.uk/project/19D7A33F-E0B6-432C-B924-70F8EA55B953

Teaching

Details on teaching interests, highlights and modules are available for Dr. Refaat Hamed as follows:

Teaching interests

Organic Chemistry, Medicinal Chemistry, Biochemistry

Teaching highlights

NMR Spectroscopy

Carbonyl Chemistry

Stereoselective Synthesis

Retrosynthesis

Professional activities

Information about education, employment and areas of particular interest for Dr. Refaat Hamed is as follows:

Employment

  • Assiut University, Egypt - Associate Professor (On leave) in the year 2016 (specified as 01/07/2016)
  • Assiut University, Egypt - Assistant Professor in the year 2011 (specified as 01/06/2011)
  •  Assiut University, Egypt - Assistant lecturer in the year 2004 (specified as 01/04/2004)
  •  Assiut University, Egypt - Demonstrator in the year 1999 (specified as 01/12/1999)
  • University of Bradford - Lecturer of Chemical Biology in the year 2018 (specified as 27/02/2018)
  • University of St Andrews - Research Fellow in the year 2016 (specified as 16/01/2016)
  • University of Illinois Urbana Champaign - Research Fellow in the year 2013 (specified as 30/05/2013)
  • University of Oxford - Research Fellow in the year 2010 (specified as 10/03/2010)

Education

  • University of Oxford - DPhil Organic Chemistry
  • Assiut University - MSc in Natural Products Chemistry
  • Assiut University - BSc in Pharmaceutical Sciences (with highest honors)

Publications

There are 29 publications involving or that are attributed to Dr. Refaat Hamed.

Peer Reviewed Journal

Dr. Refaat Hamed has 29 publications listed under peer reviewed journal.
Title Year Publication name Journal Volume Pages Authors Editors ISSN Publisher DOI Location
Self-hydroxylation of the splicing factor lysyl hydroxylase, JMJD6 2012 MedChemComm Mantri, M.;Webby, C.J.;Loik, N.D.;Hamed, R.B.;Nielsen, M.L.;McDonough, M.A.;McCullagh, J.S.O.;Böttger, A.;Schofield, C.J.;Wolf, A.; 10.1039/c1md00225b
Oxygenase-catalyzed ribosome hydroxylation occurs in prokaryotes and humans 2012 Nature Chemical Biology Ge, W.;Wolf, A.;Feng, T.;Ho, C.-H.;Sekirnik, R.;Zayer, A.;Granatino, N.;Cockman, M.E.;Loenarz, C.;Loik, N.D.;Hardy, A.P.;Claridge, T.D.W.;Hamed, R.B.;Chowdhury, R.;Gong, L.;Robinson, C.V.;Trudgian, D.C.;Jiang, M.;MacKeen, M.M.;McCullagh, J.S.;Gordiyenko, Y.;Thalhammer, A.;Yamamoto, A.;Yang, M.;Liu-Yi, P.;Zhang, Z.;Schmidt-Zachmann, M.;Kessler, B.M.;Ratcliffe, P.J.;Preston, G.M.;Coleman, M.L.;Schofield, C.J.; 10.1038/nchembio.1093
Factor-inhibiting hypoxia-inducible factor (FIH) catalyses the post-translational hydroxylation of histidinyl residues within ankyrin repeat domains 2011 FEBS Journal Yang, M.;Chowdhury, R.;Ge, W.;Hamed, R.B.;McDonough, M.A.;Claridge, T.D.W.;Kessler, B.M.;Cockman, M.E.;Ratcliffe, P.J.;Schofield, C.J.; 10.1111/j.1742-4658.2011.08022.x
Use of Methylmalonyl-CoA Epimerase in Enhancing Crotonase Stereoselectivity 2016 ChemBioChem 17 Hamed, Refaat B.; Gomez-Castellanos, J.R.; Sean Froese, D.; Krysztofinska, E.; Yue, W.W.; Schofield, C.J.
Use of Methylmalonyl-CoA Epimerase in Enhancing Crotonase Stereoselectivity 2016 ChemBioChem 17 Hamed, Refaat B.; Gomez-Castellanos, J.R.; Sean Froese, D.; Krysztofinska, E.; Yue, W.W.; Schofield, C.J.
Stereoselective preparation of lipidated carboxymethyl-proline/pipecolic acid derivatives via coupling of engineered crotonases with an alkylmalonyl-CoA synthetase 2013 Organic and Biomolecular Chemistry Hamed, R.B.;Henry, L.;Gomez-Castellanos, J.R.;Asghar, A.;Brem, J.;Claridge, T.D.W.;Schofield, C.J.; 10.1039/c3ob41525b
Crotonase catalysis enables flexible production of functionalized prolines and carbapenams 2012 Journal of the American Chemical Society Hamed, R.B.;Henry, L.;Gomez-Castellanos, J.R.;Mecinović, J.;Ducho, C.;Sorensen, J.L.;Claridge, T.D.W.;Schofield, C.J.; 10.1021/ja208318d
The enzymes of β-lactam biosynthesis 2013 Natural Product Reports Hamed, R.B.;Gomez-Castellanos, J.R.;Henry, L.;Ducho, C.;McDonough, M.A.;Schofield, C.J.; 10.1039/c2np20065a
Living GenoChemetics by hyphenating synthetic biology and synthetic chemistry in vivo 2017 Nature Communications 8 Sharma, S.V.; Tong, X.; Pubill-Ulldemolins, C.; Cartmell, C.; Bogosyan, E.J.A.; Rackham, E.J.; Marelli, E.; Hamed, Refaat B.; Goss, R.J.M.
Living GenoChemetics by hyphenating synthetic biology and synthetic chemistry in vivo 2017 Nature Communications 8 Sharma, S.V.; Tong, X.; Pubill-Ulldemolins, C.; Cartmell, C.; Bogosyan, E.J.A.; Rackham, E.J.; Marelli, E.; Hamed, Refaat B.; Goss, R.J.M.
Mechanisms and structures of crotonase superfamily enzymes - How nature controls enolate and oxyanion reactivity 2008 Cellular and Molecular Life Sciences Hamed, R.B.;Batchelar, E.T.;Clifton, I.J.;Schofield, C.J.; 10.1007/s00018-008-8082-6
The 2-Oxoglutarate-Dependent Oxygenase JMJD6 Catalyses Oxidation of Lysine Residues to give 5S-Hydroxylysine Residues 2011 ChemBioChem Mantri, M.;Loik, N.D.;Hamed, R.B.;Claridge, T.D.W.;McCullagh, J.S.O.;Schofield, C.J.; 10.1002/cbic.201000641
Synthesis of regio- and stereoselectively deuterium-labelled derivatives of l-glutamate semialdehyde for studies on carbapenem biosynthesis 2009 Organic and Biomolecular Chemistry Ducho, C.;Hamed, R.B.;Batchelar, E.T.;Sorensen, J.L.;Odell, B.;Schofield, C.J.; 10.1039/b903312b
Evidence for the slow reaction of hypoxia-inducible factor prolyl hydroxylase 2 with oxygen 2010 FEBS Journal Flashman, E.;Hoffart, L.M.;Hamed, R.B.;Bollinger Jr., J.M.;Krebs, C.;Schofield, C.J.; 10.1111/j.1742-4658.2010.07804.x
Stereoselective C-C bond formation catalysed by engineered carboxymethylproline synthases 2011 Nature Chemistry Hamed, R.B.;Gomez-Castellanos, J.R.;Thalhammer, A.;Harding, D.;Ducho, C.;Claridge, T.D.W.;Schofield, C.J.; 10.1038/nchem.1011
Photoactivable peptides for identifying enzyme-substrate and protein-protein interactions 2011 Chemical Communications Rotili, D.;Altun, M.;Hamed, R.B.;Loenarz, C.;Thalhammer, A.;Hopkinson, R.J.;Tian, Y.-M.;Ratcliffe, P.J.;Mai, A.;Kessler, B.M.;Schofield, C.J.; 10.1039/c0cc04457a
Carboxymethylproline synthase catalysed syntheses of functionalised N-heterocycles 2010 Chemical Communications Hamed, R.B.;Mecinović, J.;Ducho, C.;Claridge, T.D.W.;Schofield, C.J.; 10.1039/b924519g
Monitoring the activity of 2-oxoglutarate dependent histone demethylases by NMR spectroscopy: Direct observation of formaldehyde 2010 ChemBioChem Hopkinson, R.J.;Hamed, R.B.;Rose, N.R.;Claridge, T.D.W.;Schofield, C.J.; 10.1002/cbic.200900713
Evidence that thienamycin biosynthesis proceeds via C-5 epimerization: ThnE catalyzes the formation of (2S,5S)-trans-carboxymethylproline 2009 ChemBioChem Hamed, R.B.;Batchelar, E.T.;Mecinović, J.;Claridge, T.D.W.;Schofield, C.J.; 10.1002/cbic.200800652
Iron-mediated cleavage of C-C bonds in vicinal tricarbonyl compounds in water 2009 Angewandte Chemie - International Edition Mecinović, J.;Hamed, R.B.;Schofield, C.J.; 10.1002/anie.200806296
Thioester hydrolysis and C-C bond formation by carboxymethylproline synthase from the crotonase superfamily 2008 Angewandte Chemie - International Edition Batchelar, E.T.;Hamed, R.B.;Ducho, C.;Claridge, T.D.W.;Edelmann, M.J.;Kessler, B.;Schofield, C.J.; 10.1002/anie.200803906
Biocatalytic production of bicyclic β-lactams with three contiguous chiral centres using engineered crotonases 2019 Communications Chemistry 2 Hamed, Refaat B.; Gomez-Castellanos, J.R.; Warhaut, H.L.; Claridge, T.D.W.; Schofield, C.J.
Crotonases: Nature’s exceedingly convertible catalysts 2017 ACS Catalysis 7 Lohans, C.T.; Wang, D.Y.; Wang, J.; Hamed, Refaat B.; Schofield, C.J.
Stereoselective production of dimethyl-substituted carbapenams via engineered carbapenem biosynthesis enzymes 2017 ACS Catalysis 7 Hamed, Refaat B.; Henry, L.; Claridge, T.D.W.; Schofield, C.
A natural solution to photoprotection and isolation of the potent polyene antibiotic, marinomycin A 2019 Chemical Science 10 Christopher S. Bailey, Joseph S. Zarins-Tutt, Matthias Agbo, Hong Gao, Alberto Diego-Taboada, Maoluo Gan, Refaat B. Hamed, Emily R. Abraham, Grahame Mackenzie, P. Andrew Evans, Rebecca J. M. Goss; 10.1039/C9SC01375J
A natural solution to photoprotection and isolation of the potent polyene antibiotic, marinomycin A 2019 Chemical Science 10 Christopher S. Bailey, Joseph S. Zarins-Tutt, Matthias Agbo, Hong Gao, Alberto Diego-Taboada, Maoluo Gan, Refaat B. Hamed, Emily R. Abraham, Grahame Mackenzie, P. Andrew Evans, Rebecca J. M. Goss; 10.1039/C9SC01375J
A natural solution to photoprotection and isolation of the potent polyene antibiotic, marinomycin A 2019 Chemical Science 10 Christopher S. Bailey, Joseph S. Zarins-Tutt, Matthias Agbo, Hong Gao, Alberto Diego-Taboada, Maoluo Gan, Refaat B. Hamed, Emily R. Abraham, Grahame Mackenzie, P. Andrew Evans, Rebecca J. M. Goss; 10.1039/C9SC01375J
Studies on the selectivity of proline hydroxylases reveal new substrates including bicycles 2019 Bioorganic Chemistry 94 Tristan J. Smart, Refaat B. Hamed, Timothy D.W. Claridge, Christopher J. Schofield 0045-2068 10.1016/j.bioorg.2019.103386
Studies on the selectivity of proline hydroxylases reveal new substrates including bicycles 2019 Bioorganic Chemistry 94 Tristan J. Smart, Refaat B. Hamed, Timothy D.W. Claridge, Christopher J. Schofield 0045-2068 10.1016/j.bioorg.2019.103386