Dr. Refaat Hamed

Date

1 February 2019 to 31 January 2022

Funder

Biotechnology and Biological Sciences Research Council

http://gtr.rcuk.ac.uk/project/19D7A33F-E0B6-432C-B924-70F8EA55B953

• Assiut University Encouragement Award (1 January 2017)

• University of Oxford - DPhil Organic Chemistry
• Assiut University - BSc in Pharmaceutical Sciences (with highest honors)
• Assiut University - MSc in Natural Products Chemistry

• University of Bradford - Lecturer of Chemical Biology (27 February 2018)
• Assiut University, Egypt - Associate Professor (On leave) (1 July 2016)
• University of St Andrews - Research Fellow (16 January 2016)
• University of Illinois Urbana Champaign - Research Fellow (30 May 2013)
• Assiut University, Egypt - Assistant Professor (1 June 2011)
• University of Oxford - Research Fellow (10 March 2010)
•  Assiut University, Egypt - Assistant lecturer (1 April 2004)
•  Assiut University, Egypt - Demonstrator (1 December 1999)

• British Society for Antimicrobial Chemotherapy, Member of British Society for Antimicrobial Chemotherapy (1 April 2019)
• American Chemical Society, Member of American Chemical Society (1 December 2018)
• Biochemical Society, Member of Biochemical Society (26 October 2018)
• Royal Society of Chemistry, Member of Royal Society of Chemistry (28 January 2011)

• Biocatalytic production of bicyclic β-lactams with three contiguous chiral centres using engineered crotonases

  Hamed, Refaat B.; Gomez-Castellanos, J.R.; Warhaut, H.L.; Claridge, T.D.W.; Schofield, C.J. (2019) Communications Chemistry. 2

• Studies on the selectivity of proline hydroxylases reveal new substrates including bicycles

  Tristan J. Smart, Refaat B. Hamed, Timothy D.W. Claridge, Christopher J. Schofield (2019) Bioorganic Chemistry. 94

• A natural solution to photoprotection and isolation of the potent polyene antibiotic, marinomycin A

  Christopher S. Bailey, Joseph S. Zarins-Tutt, Matthias Agbo, Hong Gao, Alberto Diego-Taboada, Maoluo Gan, Refaat B. Hamed, Emily R. Abraham, Grahame Mackenzie, P. Andrew Evans, Rebecca J. M. Goss; (2019) Chemical Science. 10

• Stereoselective production of dimethyl-substituted carbapenams via engineered carbapenem biosynthesis enzymes

  Hamed, Refaat B.; Henry, L.; Claridge, T.D.W.; Schofield, C. (2017) ACS Catalysis. 7

• Living GenoChemetics by hyphenating synthetic biology and synthetic chemistry in vivo

  Sharma, S.V.; Tong, X.; Pubill-Ulldemolins, C.; Cartmell, C.; Bogosyan, E.J.A.; Rackham, E.J.; Marelli, E.; Hamed, Refaat B.; Goss, R.J.M. (2017) Nature Communications. 8

• Crotonases: Nature’s exceedingly convertible catalysts

  Lohans, C.T.; Wang, D.Y.; Wang, J.; Hamed, Refaat B.; Schofield, C.J. (2017) ACS Catalysis. 7

• Use of Methylmalonyl-CoA Epimerase in Enhancing Crotonase Stereoselectivity

  Hamed, Refaat B.; Gomez-Castellanos, J.R.; Sean Froese, D.; Krysztofinska, E.; Yue, W.W.; Schofield, C.J. (2016) ChemBioChem. 17

• The enzymes of β-lactam biosynthesis

  Hamed, R.B.;Gomez-Castellanos, J.R.;Henry, L.;Ducho, C.;McDonough, M.A.;Schofield, C.J.; (2013) Natural Product Reports.

• Stereoselective preparation of lipidated carboxymethyl-proline/pipecolic acid derivatives via coupling of engineered crotonases with an alkylmalonyl-CoA synthetase

  Hamed, R.B.;Henry, L.;Gomez-Castellanos, J.R.;Asghar, A.;Brem, J.;Claridge, T.D.W.;Schofield, C.J.; (2013) Organic and Biomolecular Chemistry.

• Self-hydroxylation of the splicing factor lysyl hydroxylase, JMJD6

  Mantri, M.;Webby, C.J.;Loik, N.D.;Hamed, R.B.;Nielsen, M.L.;McDonough, M.A.;McCullagh, J.S.O.;Böttger, A.;Schofield, C.J.;Wolf, A.; (2012) MedChemComm.

• Crotonase catalysis enables flexible production of functionalized prolines and carbapenams

  Hamed, R.B.;Henry, L.;Gomez-Castellanos, J.R.;Mecinović, J.;Ducho, C.;Sorensen, J.L.;Claridge, T.D.W.;Schofield, C.J.; (2012) Journal of the American Chemical Society.

• Oxygenase-catalyzed ribosome hydroxylation occurs in prokaryotes and humans

  Ge, W.;Wolf, A.;Feng, T.;Ho, C.-H.;Sekirnik, R.;Zayer, A.;Granatino, N.;Cockman, M.E.;Loenarz, C.;Loik, N.D.;Hardy, A.P.;Claridge, T.D.W.;Hamed, R.B.;Chowdhury, R.;Gong, L.;Robinson, C.V.;Trudgian, D.C.;Jiang, M.;MacKeen, M.M.;McCullagh, J.S.;Gordiyenko, Y.;Thalhammer, A.;Yamamoto, A.;Yang, M.;Liu-Yi, P.;Zhang, Z.;Schmidt-Zachmann, M.;Kessler, B.M.;Ratcliffe, P.J.;Preston, G.M.;Coleman, M.L.;Schofield, C.J.; (2012) Nature Chemical Biology.

• Photoactivable peptides for identifying enzyme-substrate and protein-protein interactions

  Rotili, D.;Altun, M.;Hamed, R.B.;Loenarz, C.;Thalhammer, A.;Hopkinson, R.J.;Tian, Y.-M.;Ratcliffe, P.J.;Mai, A.;Kessler, B.M.;Schofield, C.J.; (2011) Chemical Communications.

• The 2-Oxoglutarate-Dependent Oxygenase JMJD6 Catalyses Oxidation of Lysine Residues to give 5S-Hydroxylysine Residues

  Mantri, M.;Loik, N.D.;Hamed, R.B.;Claridge, T.D.W.;McCullagh, J.S.O.;Schofield, C.J.; (2011) ChemBioChem.

• Factor-inhibiting hypoxia-inducible factor (FIH) catalyses the post-translational hydroxylation of histidinyl residues within ankyrin repeat domains

  Yang, M.;Chowdhury, R.;Ge, W.;Hamed, R.B.;McDonough, M.A.;Claridge, T.D.W.;Kessler, B.M.;Cockman, M.E.;Ratcliffe, P.J.;Schofield, C.J.; (2011) FEBS Journal.

• Stereoselective C-C bond formation catalysed by engineered carboxymethylproline synthases

  Hamed, R.B.;Gomez-Castellanos, J.R.;Thalhammer, A.;Harding, D.;Ducho, C.;Claridge, T.D.W.;Schofield, C.J.; (2011) Nature Chemistry.

• Carboxymethylproline synthase catalysed syntheses of functionalised N-heterocycles

  Hamed, R.B.;Mecinović, J.;Ducho, C.;Claridge, T.D.W.;Schofield, C.J.; (2010) Chemical Communications.

• Evidence for the slow reaction of hypoxia-inducible factor prolyl hydroxylase 2 with oxygen

  Flashman, E.;Hoffart, L.M.;Hamed, R.B.;Bollinger Jr., J.M.;Krebs, C.;Schofield, C.J.; (2010) FEBS Journal.

• Monitoring the activity of 2-oxoglutarate dependent histone demethylases by NMR spectroscopy: Direct observation of formaldehyde

  Hopkinson, R.J.;Hamed, R.B.;Rose, N.R.;Claridge, T.D.W.;Schofield, C.J.; (2010) ChemBioChem.

• Synthesis of regio- and stereoselectively deuterium-labelled derivatives of l-glutamate semialdehyde for studies on carbapenem biosynthesis

  Ducho, C.;Hamed, R.B.;Batchelar, E.T.;Sorensen, J.L.;Odell, B.;Schofield, C.J.; (2009) Organic and Biomolecular Chemistry.

• Iron-mediated cleavage of C-C bonds in vicinal tricarbonyl compounds in water

  Mecinović, J.;Hamed, R.B.;Schofield, C.J.; (2009) Angewandte Chemie - International Edition.

• Evidence that thienamycin biosynthesis proceeds via C-5 epimerization: ThnE catalyzes the formation of (2S,5S)-trans-carboxymethylproline

  Hamed, R.B.;Batchelar, E.T.;Mecinović, J.;Claridge, T.D.W.;Schofield, C.J.; (2009) ChemBioChem.

• Mechanisms and structures of crotonase superfamily enzymes - How nature controls enolate and oxyanion reactivity

  Hamed, R.B.;Batchelar, E.T.;Clifton, I.J.;Schofield, C.J.; (2008) Cellular and Molecular Life Sciences.

• Thioester hydrolysis and C-C bond formation by carboxymethylproline synthase from the crotonase superfamily

  Batchelar, E.T.;Hamed, R.B.;Ducho, C.;Claridge, T.D.W.;Edelmann, M.J.;Kessler, B.;Schofield, C.J.; (2008) Angewandte Chemie - International Edition.