Dr. Refaat Hamed,Assistant Professor
Information about Dr. Refaat Hamed at the University of Bradford.
- School of Chemistry & Biosciences
(Faculty of Life Sciences) - Email:
- r.farghal@bradford.ac.uk
- Telephone:
- +44 1274 232371
Biography
I have always been fascinated by the way nature morphs simple building blocks to create more complex natural cures. This fascination shaped my career, which started by the isolation, structure elucidation and biological evaluation of various classes of phytochemicals, with the focus being iridoid glycosides (MSc studies). I then moved on to pursue my D.Phil. studies at University of Oxford, where I learned the modern aspects of (b-lactam antibiotics) biosynthesis, molecular biology and enzymology (structural and mechanistic). During my D.Phil. and postdoctoral studies time, I have developed myself into a rational-design protein engineer. Challenging chemical transformations and forcing enzymes, via engineering, to catalyse these reactions, with exquisite regio- and stereoselectivity, for biocatalytic purposes, is one of the main themes of my research. Extending my research from engineering enzymes to redesign of biosynthetic pathways to produce unnatural products of pharmaceutical interest is another objective for my research. As a teacher, I do strive to be well-informed, interactive and inspiring. One of my primary goals of teaching is to prepare both undergraduate and graduate students for a successful career.
Research
Natural products discovery, Biosynthesis of natural products, Biocatalysis, Enzymology, Protein Engineering and Synthetic Biology
Teaching
Details on teaching interests, highlights and modules are available for Dr. Refaat Hamed as follows:
Teaching interests
Organic Chemistry, Medicinal Chemistry, Biochemistry
Professional activities
Information about education, employment and areas of particular interest for Dr. Refaat Hamed is as follows:
Employment
- Assiut University, Egypt - Assistant lecturer in the year 2004 (specified as 01/04/2004)
- University of Bradford - Lecturer of Chemical Biology in the year 2018 (specified as 27/02/2018)
- Assiut University, Egypt - Associate Professor (On leave) in the year 2016 (specified as 01/07/2016)
- University of Illinois Urbana Champaign - Research Fellow in the year 2013 (specified as 30/05/2013)
- Assiut University, Egypt - Assistant Professor in the year 2011 (specified as 01/06/2011)
- University of St Andrews - Research Fellow in the year 2016 (specified as 16/01/2016)
- University of Oxford - Research Fellow in the year 2010 (specified as 10/03/2010)
- Assiut University, Egypt - Demonstrator in the year 1999 (specified as 01/12/1999)
Education
- University of Oxford - DPhil Organic Chemistry
- Assiut University - BSc in Pharmaceutical Sciences (with highest honors)
- Assiut University - MSc in Natural Products Chemistry
- Assiut University Encouragement Award in the year 01-JAN-17 (specified as 01/01/2017)
- British Society for Antimicrobial Chemotherapy, Member of British Society for Antimicrobial Chemotherapy in the year 2019 (specified as 01/04/2019)
- Royal Society of Chemistry, Member of Royal Society of Chemistry in the year 2011 (specified as 28/01/2011)
- Biochemical Society, Member of Biochemical Society in the year 2018 (specified as 26/10/2018)
- American Chemical Society, Member of American Chemical Society in the year 2018 (specified as 01/12/2018)
Publications
There are 24 publications involving or that are attributed to Dr. Refaat Hamed.
Peer Reviewed Journal
Title | Year | Publication name | Journal | Volume | Pages | Authors | Editors | ISSN | Publisher | DOI | Location |
---|---|---|---|---|---|---|---|---|---|---|---|
Stereoselective production of dimethyl-substituted carbapenams via engineered carbapenem biosynthesis enzymes | 2017 | ACS Catalysis | 7 | Hamed, Refaat B.; Henry, L.; Claridge, T.D.W.; Schofield, C. | |||||||
Crotonases: Nature’s exceedingly convertible catalysts | 2017 | ACS Catalysis | 7 | Lohans, C.T.; Wang, D.Y.; Wang, J.; Hamed, Refaat B.; Schofield, C.J. | |||||||
Biocatalytic production of bicyclic β-lactams with three contiguous chiral centres using engineered crotonases | 2019 | Communications Chemistry | 2 | Hamed, Refaat B.; Gomez-Castellanos, J.R.; Warhaut, H.L.; Claridge, T.D.W.; Schofield, C.J. | |||||||
Studies on the selectivity of proline hydroxylases reveal new substrates including bicycles | 2019 | Bioorganic Chemistry | 94 | Tristan J. Smart, Refaat B. Hamed, Timothy D.W. Claridge, Christopher J. Schofield | 0045-2068 | 10.1016/j.bioorg.2019.103386 | |||||
A natural solution to photoprotection and isolation of the potent polyene antibiotic, marinomycin A | 2019 | Chemical Science | 10 | Christopher S. Bailey, Joseph S. Zarins-Tutt, Matthias Agbo, Hong Gao, Alberto Diego-Taboada, Maoluo Gan, Refaat B. Hamed, Emily R. Abraham, Grahame Mackenzie, P. Andrew Evans, Rebecca J. M. Goss; | 10.1039/C9SC01375J | ||||||
Living GenoChemetics by hyphenating synthetic biology and synthetic chemistry in vivo | 2017 | Nature Communications | 8 | Sharma, S.V.; Tong, X.; Pubill-Ulldemolins, C.; Cartmell, C.; Bogosyan, E.J.A.; Rackham, E.J.; Marelli, E.; Hamed, Refaat B.; Goss, R.J.M. | |||||||
Use of Methylmalonyl-CoA Epimerase in Enhancing Crotonase Stereoselectivity | 2016 | ChemBioChem | 17 | Hamed, Refaat B.; Gomez-Castellanos, J.R.; Sean Froese, D.; Krysztofinska, E.; Yue, W.W.; Schofield, C.J. | |||||||
The enzymes of β-lactam biosynthesis | 2013 | Natural Product Reports | Hamed, R.B.;Gomez-Castellanos, J.R.;Henry, L.;Ducho, C.;McDonough, M.A.;Schofield, C.J.; | 10.1039/c2np20065a | |||||||
Stereoselective preparation of lipidated carboxymethyl-proline/pipecolic acid derivatives via coupling of engineered crotonases with an alkylmalonyl-CoA synthetase | 2013 | Organic and Biomolecular Chemistry | Hamed, R.B.;Henry, L.;Gomez-Castellanos, J.R.;Asghar, A.;Brem, J.;Claridge, T.D.W.;Schofield, C.J.; | 10.1039/c3ob41525b | |||||||
Self-hydroxylation of the splicing factor lysyl hydroxylase, JMJD6 | 2012 | MedChemComm | Mantri, M.;Webby, C.J.;Loik, N.D.;Hamed, R.B.;Nielsen, M.L.;McDonough, M.A.;McCullagh, J.S.O.;Böttger, A.;Schofield, C.J.;Wolf, A.; | 10.1039/c1md00225b | |||||||
Crotonase catalysis enables flexible production of functionalized prolines and carbapenams | 2012 | Journal of the American Chemical Society | Hamed, R.B.;Henry, L.;Gomez-Castellanos, J.R.;MecinoviÄ, J.;Ducho, C.;Sorensen, J.L.;Claridge, T.D.W.;Schofield, C.J.; | 10.1021/ja208318d | |||||||
Oxygenase-catalyzed ribosome hydroxylation occurs in prokaryotes and humans | 2012 | Nature Chemical Biology | Ge, W.;Wolf, A.;Feng, T.;Ho, C.-H.;Sekirnik, R.;Zayer, A.;Granatino, N.;Cockman, M.E.;Loenarz, C.;Loik, N.D.;Hardy, A.P.;Claridge, T.D.W.;Hamed, R.B.;Chowdhury, R.;Gong, L.;Robinson, C.V.;Trudgian, D.C.;Jiang, M.;MacKeen, M.M.;McCullagh, J.S.;Gordiyenko, Y.;Thalhammer, A.;Yamamoto, A.;Yang, M.;Liu-Yi, P.;Zhang, Z.;Schmidt-Zachmann, M.;Kessler, B.M.;Ratcliffe, P.J.;Preston, G.M.;Coleman, M.L.;Schofield, C.J.; | 10.1038/nchembio.1093 | |||||||
The 2-Oxoglutarate-Dependent Oxygenase JMJD6 Catalyses Oxidation of Lysine Residues to give 5S-Hydroxylysine Residues | 2011 | ChemBioChem | Mantri, M.;Loik, N.D.;Hamed, R.B.;Claridge, T.D.W.;McCullagh, J.S.O.;Schofield, C.J.; | 10.1002/cbic.201000641 | |||||||
Stereoselective C-C bond formation catalysed by engineered carboxymethylproline synthases | 2011 | Nature Chemistry | Hamed, R.B.;Gomez-Castellanos, J.R.;Thalhammer, A.;Harding, D.;Ducho, C.;Claridge, T.D.W.;Schofield, C.J.; | 10.1038/nchem.1011 | |||||||
Factor-inhibiting hypoxia-inducible factor (FIH) catalyses the post-translational hydroxylation of histidinyl residues within ankyrin repeat domains | 2011 | FEBS Journal | Yang, M.;Chowdhury, R.;Ge, W.;Hamed, R.B.;McDonough, M.A.;Claridge, T.D.W.;Kessler, B.M.;Cockman, M.E.;Ratcliffe, P.J.;Schofield, C.J.; | 10.1111/j.1742-4658.2011.08022.x | |||||||
Photoactivable peptides for identifying enzyme-substrate and protein-protein interactions | 2011 | Chemical Communications | Rotili, D.;Altun, M.;Hamed, R.B.;Loenarz, C.;Thalhammer, A.;Hopkinson, R.J.;Tian, Y.-M.;Ratcliffe, P.J.;Mai, A.;Kessler, B.M.;Schofield, C.J.; | 10.1039/c0cc04457a | |||||||
Carboxymethylproline synthase catalysed syntheses of functionalised N-heterocycles | 2010 | Chemical Communications | Hamed, R.B.;MecinoviÄ, J.;Ducho, C.;Claridge, T.D.W.;Schofield, C.J.; | 10.1039/b924519g | |||||||
Evidence for the slow reaction of hypoxia-inducible factor prolyl hydroxylase 2 with oxygen | 2010 | FEBS Journal | Flashman, E.;Hoffart, L.M.;Hamed, R.B.;Bollinger Jr., J.M.;Krebs, C.;Schofield, C.J.; | 10.1111/j.1742-4658.2010.07804.x | |||||||
Monitoring the activity of 2-oxoglutarate dependent histone demethylases by NMR spectroscopy: Direct observation of formaldehyde | 2010 | ChemBioChem | Hopkinson, R.J.;Hamed, R.B.;Rose, N.R.;Claridge, T.D.W.;Schofield, C.J.; | 10.1002/cbic.200900713 | |||||||
Synthesis of regio- and stereoselectively deuterium-labelled derivatives of l-glutamate semialdehyde for studies on carbapenem biosynthesis | 2009 | Organic and Biomolecular Chemistry | Ducho, C.;Hamed, R.B.;Batchelar, E.T.;Sorensen, J.L.;Odell, B.;Schofield, C.J.; | 10.1039/b903312b | |||||||
Iron-mediated cleavage of C-C bonds in vicinal tricarbonyl compounds in water | 2009 | Angewandte Chemie - International Edition | MecinoviÄ, J.;Hamed, R.B.;Schofield, C.J.; | 10.1002/anie.200806296 | |||||||
Evidence that thienamycin biosynthesis proceeds via C-5 epimerization: ThnE catalyzes the formation of (2S,5S)-trans-carboxymethylproline | 2009 | ChemBioChem | Hamed, R.B.;Batchelar, E.T.;MecinoviÄ, J.;Claridge, T.D.W.;Schofield, C.J.; | 10.1002/cbic.200800652 | |||||||
Mechanisms and structures of crotonase superfamily enzymes - How nature controls enolate and oxyanion reactivity | 2008 | Cellular and Molecular Life Sciences | Hamed, R.B.;Batchelar, E.T.;Clifton, I.J.;Schofield, C.J.; | 10.1007/s00018-008-8082-6 | |||||||
Thioester hydrolysis and C-C bond formation by carboxymethylproline synthase from the crotonase superfamily | 2008 | Angewandte Chemie - International Edition | Batchelar, E.T.;Hamed, R.B.;Ducho, C.;Claridge, T.D.W.;Edelmann, M.J.;Kessler, B.;Schofield, C.J.; | 10.1002/anie.200803906 |