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researcher

Mark Sutherland

Lecturer in Biochemistry

Faculty/Dept/School School of Chemistry and Bioscience
(Faculty of Life Sciences)
Emailm.sutherland@bradford.ac.uk
Telephone +441274 236215

Biography

Dr Mark Sutherland is a Lecturer in Biochemistry in the School of Chemistry and Biosciences, University of Bradford. After completing his MSc in Microbiology at the University of Stellenbosch, South Africa he worked for a few years as a school teacher and University Lecturer. He was offered a PhD at the Charite – Campus Benjamin Franklin, Berlin, Germany where he received his doctorate in Medical Biochemistry. He then worked for a number of years as a PDRA in Berlin in the field of immunotoxins. This work resulted in a number of publications, review articles and book chapters. In 2005 he moved to the Institute of Cancer Therapeutics at the University of Bradford. He carried out research for a number of projects within the Institute to generate proof-of-concept data for grant and patent applications. He also taught laboratory skills to MSc and PhD students, and lectured undergraduate and MSc students. His research was in the field of integrins as a target for new anti-cancer drugs in prostate cancer and melanoma, as well as the role of sialyltransferases in neuroblastoma. The research involves a wide range of methodologies including generating lentivirus knockout models (shRNA), development of an ELISA for biomarker analysis, protein expression/purification using GST-tagged protein in E.coli and luciferase reporter assays. This work has led to numerous publications, and successful grant applications. Dr Sutherland has vast experience in an array of Molecular Biology techniques including gene cloning, transformation/transfection, Western blotting, PCR and qPCR; as well as managing the Molecular Biology Laboratory. He started his current role as Lecturer in January 2019.

Professional activities

  • 01-JAN-00: Charite Campus Benjamin Franklin - PhD (rer med)
  • 01-JAN-93: University of Stellenbosch, South Africa - MSc
  • 01-JAN-89: University of Stellenbosch, South Africa - BSc (Hon)
  • 01-JAN-88: University of Cape Town, South Africa - BSc
  • 01-NOV-05: University of Bradford - Senior Experimental Officer
  • 01-NOV-00: Charite - Klinikum Benjamin Franklin - PDRA
  • 01-JAN-95: University of Fort Hare, South Africa - Lecturer in Biochemistry and Microbiology
  • 01-JAN-94: Cape Education Departmant - Science and Mathematics teacher
  • 01-JAN-93: University of Stellenbosch, South Africa - Research Assistant
  • 01-JAN-14: Faculty of Life Sciences Genetically Modified Organisms,
  • German

Publications

Peer Reviewed Journal
TitleExploring and Exploiting Acceptor Preferences of the Human Polysialyltransferases as a Basis for an Inhibitor Screen (2017)
AuthorsEhrit, J.; Keys, T.G.; Sutherland, Mark H.; Wolf, S.; Meier, C.; Falconer, Robert A.; Gerardy-Schahn, R.
JournalChemBioChem
DOI10.1002/cbic.201700157
 
TitleAn optimised assay for quantitative, high-throughput analysis of polysialyltransferase activity (2016)
AuthorsElkashef, Sara M.; Sutherland, Mark H.; Patterson, Laurence H.; Loadman, Paul M.; Falconer, Robert A.
JournalANALYST (PRINT)
DOI10.1039/c6an01073c
 
TitleProbing cytochrome P450-mediated activation with a truncated azinomycin analogue (2015)
AuthorsVinader, Victoria; Sadiq, Maria; Sutherland, Mark H.; Huang, M.Y.; Loadman, Paul M.; Elsalem, Lina M.I.; Shnyder, Steven D.; Cui, H.J.; Afarinkia, Kamyar; Searcey, M.; Patterson, Laurence H.; Pors, Klaus
 
TitlePharmacological Inhibition of polysialyltransferase ST8SiaII Modulates Tumour Cell Migration (2013)
AuthorsAl-Saraireh, Yousef M.J.; Sutherland, Mark H.; Springett, Bradley R.; Freiberger, F.; Ribeiro Morais, Goreti; Loadman, Paul M.; Errington, R.J.; Smith, P.J.; Fukuda, M.; Gerardy-Schahn, R.; Patterson, Laurence H.; Shnyder, Steven D.; Falconer, Robert A.
JournalPLoS ONE
DOI10.1371/journal.pone.0073366
 
TitleRe-engineering of the Duocarmycin Structural Architecture Enables Bioprecursor Development Targeting CYP1A1 and CYP2W1 for Biological Activity (2013)
AuthorsSheldrake, Helen M.; Travica, S.; Johansson, I.; Loadman, Paul M.; Sutherland, Mark H.; Elsalem, Lina M.I.; Illingworth, Nicola A.; Cresswell, Alexander J.; Reuillon, Tristan; Shnyder, Steven D.; Mkrtchian, S.; Searcey, M.; Ingelman-Sundberg, M.; Patterson, Laurence H.; Pors, Klaus
 
TitleAntitumor activity of a duocarmycin analogue rationalized to be metabolically activated by cytochrome P450 1A1 in human transitional cell carcinoma of the bladder (2013)
AuthorsSutherland, Mark H.; Gill, Jason H.; Loadman, Paul M.; Laye, Jonathan P.; Sheldrake, Helen M.; Illingworth, Nicola A.; Alandas, Mohammed N.; Cooper, Patricia A.; Searcey, M.; Pors, Klaus; Shnyder, Steven D.; Patterson, Laurence H.
 
TitleModification of the duocarmycin pharmacophore enables CYP1A1 targeting for biological activity (2011)
AuthorsPors, Klaus; Loadman, Paul M.; Shnyder, Steven D.; Sutherland, Mark H.; Sheldrake, Helen M.; Guino, M.; Kiakos, K.; Hartley, J.A.; Searcey, M.; Patterson, Laurence H.
 
TitleA lysine-free mutant of epidermal growth factor as targeting moiety of a targeted toxin (2011)
AuthorsBachran C.;Schneider S.;Riese S.;Bachran D.;Urban R.;Schellmann N.;Zahn C.;Sutherland M.;Fuchs H.
JournalLife Sciences
DOI10.1016/j.lfs.2010.11.012
 
TitleImproved preparation and detection of cytochrome P450 isoforms using MS methods (2010)
AuthorsSutton C.;Sutherland M.;Shnyder S.;Patterson L.
JournalProteomics
DOI10.1002/pmic.200900489
 
TitleQuantification of diphtheria toxin-mediated ADP-ribosylation in a solid-phase assay (2007)
AuthorsBachran C.;Sutherland M.;Bachran D.;Fuchs H.
JournalClinical Chemistry
DOI10.1373/clinchem.2007.085365
 
TitleThe saponin-mediated enhanced uptake of targeted saporin-based drugs is strongly dependent on the saponin structure (2006)
AuthorsBachran C.;Sutherland M.;Heisler I.;Hebestreit P.;Melzig M.;Fuchs H.
JournalExperimental Biology and Medicine
DOI10.1177/153537020623100407
 
Book Chapter
TitlePreclinical Studies of Saponins for Tumor Therapy (2015)
AuthorsBachran C.;Bachran S.;Sutherland M.;Bachran D.;Fuchs H.
JournalRecent Advances in Medicinal Chemistry
DOI10.1016/B978-0-12-803961-8.50009-9
 
Other Publication
Other typeReviews
TitleRGD-binding integrins in prostate cancer: Expression patterns and therapeutic prospects against bone metastasis (2012)
AuthorsSutherland M.;Gordon A.;Shnyder S.;Patterson L.;Sheldrake H.
JournalCancers
DOI10.3390/cancers4041106
 
Other typeReviews
TitleSaponins as tool for improved targeted tumor therapies (2009)
AuthorsFuchs H.;Bachran D.;Panjideh H.;Schellmann N.;Weng A.;Melzig M.;Sutherland M.;Bachran C.
JournalCurrent Drug Targets
DOI10.2174/138945009787354584
 
Other typeReviews
TitleSaponins in tumor therapy (2008)
AuthorsBachran C.;Bachran S.;Sutherland M.;Bachran D.;Fuchs H.
JournalMini-Reviews in Medicinal Chemistry
DOI10.2174/138955708784534445