Practical Skills in Pharmacology Research

Module code: INC7018-B

Module Aims       

To provide a comprehensive understanding of selected important advanced pharmacology techniques; to develop student autonomy in learning and to develop research skills as well as enhance written communication skills.

Outline Syllabus   

Theory and practice of basic and advanced laboratory techniques:

Introduction to good laboratory and clinical practice. Sample preparation for bio-analysis. Analytical biochemical techniques: separation methods, GC, HPLC, FPLC chromatography (ion, gel, affinity), electrophoresis, mass spectrometry. Methods to study cells: microscopy, cell culture, cell proliferation, identification of cell markers and cell purification by FACS. Recombinant DNA techniques include: Polymerase Chain Reaction (PCR), Northern & Southern blotting, molecular analysis of gene expression (proteomics & transgenic approaches). Writing of drug evaluation, molecular biology and pharmacology reports. Attendance at seminars and production of a summary (reflective statement) of two of the topics, one in each Semester.

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