Research and Analytical Methods

Module code: BIS7006-B

Module Aims       

To provide a comprehensive understanding of selected important advanced biomedical techniques; to develop student autonomy in learning and to develop critical insight into research skills and laboratory techniques as well as enhance written communication skills.

Outline Syllabus   

Advanced biomedical techniques: Introduction to biomedical analysis; Good laboratory and clinical practice; Sample preparation for bioanalysis. Analytical biochemical techniques: separation methods, GLC, HPLC, FPLC chromatography (ion, gel, affinity), electrophoresis, mass spectrometry. Methods to study cells: microscopy, cell culture, cell proliferation, identification of cell markers & cell purification by FACS. Recombinant DNA techniques: Concepts of gene cloning and its impact on research and biotechnology. RNA techniques; isolation of RNA, cDNA synthesis, Polymerase Chain Reaction (PCR, real-time PCR), molecular analysis of gene expression (proteomics, microarray, Next generation sequencing); gene functions analysis (transgenic approaches, RNA silencing). Attendance at Postgraduate Seminars and production of summary of topic.

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